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ab 2927413  (Proteintech)


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    Proteintech ab 2927413
    Ab 2927413, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab+2927413/HCN4+Antibody/pmc11781962__mmc9-723-17-25
    Average 93 stars, based on 13 article reviews
    ab 2927413 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. Animal experiments were approved by the institutional IACUC at Broad Institute.

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAscope probe: Cempi2-rn [C2] ACDbio 897621-C2 RNAscope probe: Bmp6-rn [C1] ACDbio 510271 RNAscope probe: Nppa-rn [C3] ACDbio 897601-C3 RNAscope probe: Apod-rn [C1] ACDbio 1071791-C1 RNAscope probe: Dcn-rn [C2] ACDbio 524281-C2 RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 Deposited data GEO https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE280111 GSE280111 Single Cell Portal https://singlecell.broadinstitute.org/ single_cell/study/SCP2828 SCP2828 Experimental models: Organisms/strains Male Wistar IGS Rat Details Charles River # 003 Software and algorithms Python v3.7.9 https://www.python.org/ CellRanger v3.0.2 https://github.com/10XGenomics/cellranger StringTie v2.1.4 https://github.com/gpertea/stringtie scrinvex https://github.com/getzlab/scrinvex cutadapt v1.18 https://github.com/marcelm/cutadapt CellBender v0.2.1 https://github.com/broadinstitute/CellBender scanpy v1.8.0 https://github.com/scverse/scanpy squidpy v1.0.0 https://github.com/scverse/squidpy scrublet v0.2.1 https://github.com/swolock/scrublet umap-learn v0.4.6 https://github.com/lmcinnes/umap scvi-tools v0.6.5 https://github.com/scverse/scvi-tools jupyter v1.0.0 https://jupyter.org/ ndd v1.7.1 https://github.com/simomarsili/ndd scikit-learn v0.23.2 https://github.com/scikit-learn/scikit-learn R v4.4.1 https://www.r-project.org/ limma v3.60.4 https://bioinf.wehi.edu.au/limma/ fgsea v1.30.0 https://github.com/ctlab/fgsea MSigDB v7.1 https://www.gsea-msigdb.org/gsea/msigdb/ Seurat v3 https://satijalab.org/seurat/ ImageJ 1.53k http://imagej.nih.gov/ij LAS-X software (microscope) Leica Other TCS SP8 X White Light Laser Confocal Microscope Leica Cryostat Leica CM1950 pluriStrainer 10uM Cell Strainer PluriSelect 43-50010-50 pluriStrainer 40uM Cell Strainer PluriSelect 43-50040-50 Resource ll OPEN ACCESS

    Single Cell:

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. Animal experiments were approved by the institutional IACUC at Broad Institute.

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAscope probe: Cempi2-rn [C2] ACDbio 897621-C2 RNAscope probe: Bmp6-rn [C1] ACDbio 510271 RNAscope probe: Nppa-rn [C3] ACDbio 897601-C3 RNAscope probe: Apod-rn [C1] ACDbio 1071791-C1 RNAscope probe: Dcn-rn [C2] ACDbio 524281-C2 RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 Deposited data GEO https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE280111 GSE280111 Single Cell Portal https://singlecell.broadinstitute.org/ single_cell/study/SCP2828 SCP2828 Experimental models: Organisms/strains Male Wistar IGS Rat Details Charles River # 003 Software and algorithms Python v3.7.9 https://www.python.org/ CellRanger v3.0.2 https://github.com/10XGenomics/cellranger StringTie v2.1.4 https://github.com/gpertea/stringtie scrinvex https://github.com/getzlab/scrinvex cutadapt v1.18 https://github.com/marcelm/cutadapt CellBender v0.2.1 https://github.com/broadinstitute/CellBender scanpy v1.8.0 https://github.com/scverse/scanpy squidpy v1.0.0 https://github.com/scverse/squidpy scrublet v0.2.1 https://github.com/swolock/scrublet umap-learn v0.4.6 https://github.com/lmcinnes/umap scvi-tools v0.6.5 https://github.com/scverse/scvi-tools jupyter v1.0.0 https://jupyter.org/ ndd v1.7.1 https://github.com/simomarsili/ndd scikit-learn v0.23.2 https://github.com/scikit-learn/scikit-learn R v4.4.1 https://www.r-project.org/ limma v3.60.4 https://bioinf.wehi.edu.au/limma/ fgsea v1.30.0 https://github.com/ctlab/fgsea MSigDB v7.1 https://www.gsea-msigdb.org/gsea/msigdb/ Seurat v3 https://satijalab.org/seurat/ ImageJ 1.53k http://imagej.nih.gov/ij LAS-X software (microscope) Leica Other TCS SP8 X White Light Laser Confocal Microscope Leica Cryostat Leica CM1950 pluriStrainer 10uM Cell Strainer PluriSelect 43-50010-50 pluriStrainer 40uM Cell Strainer PluriSelect 43-50040-50 Resource ll OPEN ACCESS

    Multiplex Assay:

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. Animal experiments were approved by the institutional IACUC at Broad Institute.

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAscope probe: Cempi2-rn [C2] ACDbio 897621-C2 RNAscope probe: Bmp6-rn [C1] ACDbio 510271 RNAscope probe: Nppa-rn [C3] ACDbio 897601-C3 RNAscope probe: Apod-rn [C1] ACDbio 1071791-C1 RNAscope probe: Dcn-rn [C2] ACDbio 524281-C2 RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 Deposited data GEO https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE280111 GSE280111 Single Cell Portal https://singlecell.broadinstitute.org/ single_cell/study/SCP2828 SCP2828 Experimental models: Organisms/strains Male Wistar IGS Rat Details Charles River # 003 Software and algorithms Python v3.7.9 https://www.python.org/ CellRanger v3.0.2 https://github.com/10XGenomics/cellranger StringTie v2.1.4 https://github.com/gpertea/stringtie scrinvex https://github.com/getzlab/scrinvex cutadapt v1.18 https://github.com/marcelm/cutadapt CellBender v0.2.1 https://github.com/broadinstitute/CellBender scanpy v1.8.0 https://github.com/scverse/scanpy squidpy v1.0.0 https://github.com/scverse/squidpy scrublet v0.2.1 https://github.com/swolock/scrublet umap-learn v0.4.6 https://github.com/lmcinnes/umap scvi-tools v0.6.5 https://github.com/scverse/scvi-tools jupyter v1.0.0 https://jupyter.org/ ndd v1.7.1 https://github.com/simomarsili/ndd scikit-learn v0.23.2 https://github.com/scikit-learn/scikit-learn R v4.4.1 https://www.r-project.org/ limma v3.60.4 https://bioinf.wehi.edu.au/limma/ fgsea v1.30.0 https://github.com/ctlab/fgsea MSigDB v7.1 https://www.gsea-msigdb.org/gsea/msigdb/ Seurat v3 https://satijalab.org/seurat/ ImageJ 1.53k http://imagej.nih.gov/ij LAS-X software (microscope) Leica Other TCS SP8 X White Light Laser Confocal Microscope Leica Cryostat Leica CM1950 pluriStrainer 10uM Cell Strainer PluriSelect 43-50010-50 pluriStrainer 40uM Cell Strainer PluriSelect 43-50040-50 Resource ll OPEN ACCESS

    RNAscope:

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. Animal experiments were approved by the institutional IACUC at Broad Institute.

    Article Title: Transcriptional profile of the rat cardiovascular system at single-cell resolution
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acta2 Sigma Cat# A5228; AB_262054; RRID: AB_262054 Myh11 Abcamm Cat# Ab224804; AB_2927413; RRID: AB_2927413 Hcn4 ThermoFisher PA5-111793; AB_2857202 Postn Proteintech Cat# 66491-1-Ig; AB_2881856; RRID: AB_2881856 Myom1 DSHB B4; AB_760349 Mpz Abcam Cat# Ab183868; AB_2895675; RRID: AB_2895675 Cnn1 Abcam Ab233854 Cdh6 Cell Signaling Cat # 48111; AB_2799334; RRID: AB_2799334 Pcm1 Abcam Cat # Ab72443; AB_1269694; RRID: AB_1269694 Donkey anti-Rabbit Alexa488 ThermoFisher Cat# A-21206; AB_2535792; RRID: AB_2535792 Donkey anti-Mouse Alexa568 ThermoFisher Cat# A-10037; AB_11180865; RRID: AB_11180865 Donkey anti-Goat Alexa647 ThermoFisher Cat# A-21447; AB_2535864; RRID: AB_2535864 DAPI ThermoFisher D1306; AB_2629482 Biological samples Male Wistar IGS Rat Details Charles River # 003 Chemicals, peptides, and recombinant proteins Hepes Sigma 54457 Sucrose Sigma 84097 Magnesium Chloride Sigma M2670 Potassium Chloride Sigma P5405 Igepal-630 Sigma 18896 Bovine Serum Albumin Sigma 126609 Critical commercial assays 10x single-cell 3’ v2 chemistry 10x Genomics ChromiumTM Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns 10x Genomics PN-120237 RNAscopeTM Multiplex Fluorescent Reagent Kit v2 ACDbio #323100 RNAscopeTM 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2 ACDbio #323120 Opal 520 Akoya Biosciences PN FP1487001KT Opal 570 Akoya Biosciences PN FP1488001KT Opal 620 Akoya Biosciences PN FP1495001KT Opal 690 Akoya Biosciences PN FP1497001KT RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 RNAscope probe: Flt1-rn [C4] ACDbio 435701-C4 RNAscope probe: Pappa1-rn [C3] ACDbio 1108071-C3 RNAscope probe: Tspan11-rn [C4] ACDbio 1108081-C4 RNAscope probe: Pdgfra-rn [C2] ACDbio 449601-C2 RNAscope probe: Adamts16-rn [C1] ACDbio 1108061-C1 RNAscope probe: Cacna1a-rn [C4] ACDbio 1085691-C4 (Continued on next page) Cell Reports 44, 115091, January 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAscope probe: Cempi2-rn [C2] ACDbio 897621-C2 RNAscope probe: Bmp6-rn [C1] ACDbio 510271 RNAscope probe: Nppa-rn [C3] ACDbio 897601-C3 RNAscope probe: Apod-rn [C1] ACDbio 1071791-C1 RNAscope probe: Dcn-rn [C2] ACDbio 524281-C2 RNAscope probe: Ptprb-rn [C3] ACDbio 837011-C3 RNAscope probe: Vwf-rn [C2] ACDbio 413401-C2 Deposited data GEO https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE280111 GSE280111 Single Cell Portal https://singlecell.broadinstitute.org/ single_cell/study/SCP2828 SCP2828 Experimental models: Organisms/strains Male Wistar IGS Rat Details Charles River # 003 Software and algorithms Python v3.7.9 https://www.python.org/ CellRanger v3.0.2 https://github.com/10XGenomics/cellranger StringTie v2.1.4 https://github.com/gpertea/stringtie scrinvex https://github.com/getzlab/scrinvex cutadapt v1.18 https://github.com/marcelm/cutadapt CellBender v0.2.1 https://github.com/broadinstitute/CellBender scanpy v1.8.0 https://github.com/scverse/scanpy squidpy v1.0.0 https://github.com/scverse/squidpy scrublet v0.2.1 https://github.com/swolock/scrublet umap-learn v0.4.6 https://github.com/lmcinnes/umap scvi-tools v0.6.5 https://github.com/scverse/scvi-tools jupyter v1.0.0 https://jupyter.org/ ndd v1.7.1 https://github.com/simomarsili/ndd scikit-learn v0.23.2 https://github.com/scikit-learn/scikit-learn R v4.4.1 https://www.r-project.org/ limma v3.60.4 https://bioinf.wehi.edu.au/limma/ fgsea v1.30.0 https://github.com/ctlab/fgsea MSigDB v7.1 https://www.gsea-msigdb.org/gsea/msigdb/ Seurat v3 https://satijalab.org/seurat/ ImageJ 1.53k http://imagej.nih.gov/ij LAS-X software (microscope) Leica Other TCS SP8 X White Light Laser Confocal Microscope Leica Cryostat Leica CM1950 pluriStrainer 10uM Cell Strainer PluriSelect 43-50010-50 pluriStrainer 40uM Cell Strainer PluriSelect 43-50040-50 Resource ll OPEN ACCESS



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